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TOOLSilent LentiBooster (500X) is a novel cocktail of small molecules that can enhance viral production and is a powerful, broadly applicable reagent for effective virus packaging. TOOLSilent LentiBooster stably regulates the viral RNA packaging on the transcriptional level, which can enhance production of either retro- or lenti-viral particles up to 10 fold. The easy-to-use protocol makes TOOLSilent LentiBooster well-suited for various scales of virus packaging.